Figure 1.

Immunoblot analysis of dinitrogenase and dinitrogenase reductase synthesis in Rs. rubrum. The gel blot was reacted with antiserum to Rs. rubrum dinitrogenase reductase (A) and dinitrogenase (B). Samples were of crude extracts from the wild-type grown on malate and ammonium sulfate (lanes 2), malate and glutamate (lanes 4), and acetate and ammonium sulfate (lanes 6); strain I-19 grown on malate and ammonium sulfate (lanes 3), malate and glutamate (lanes 5), and acetate and ammonium sulfate (lanes 7); strain I-19 (pJG106) grown on malate and ammonium sulfate (lanes 8); and strain I-32, grown on malate and ammonium sulfate (lanes 9). A prestained molecular weight marker of 34.9 kDa (lanes 1) is indicated by the left arrow in the margin and the position of the calculated molecular weights (34.6 and 36.7 kDa) of the subunits of dinitrogenase reductase, presumably modified and unmodified (10), are indicated by the arrows in the right margin.