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. 1996 Dec 10;93(25):14515–14520. doi: 10.1073/pnas.93.25.14515

Figure 2.

Figure 2

Immunoblot analysis of dinitrogenase and dinitrogenase reductase synthesis in R. sphaeroides. Antisera to dinitrogenase (A and B) and dinitrogenase reductase (C and D) were used in immunoblots of samples from R. sphaeroides grown in a malate medium using ammonium sulfate (A and C) and glutamate (B and D) as the nitrogen source: wild-type, strain HR grown in the absence (lanes 2) or presence (lanes 3) of DMSO as electron acceptor; strain 16PHC in the absence (lanes 4) or presence (lanes 5) of DMSO; strain PHG, a RubisCO− derivative of 16PHC which does not use CO2 as electron acceptor (lanes 6); strain 16 plus DMSO (lanes 7); strain 16PHCΩ plus DMSO (lanes 8); strain A25 plus DMSO (lanes 9); and strain 1312 (lanes 10). Prestained molecular weight markers (lanes 1) and approximate molecular weights of dinitrogenase and dinitrogenase reductase are shown in the right margin.