Figure 5.

Protein degradation from HA-trapped Hsp90 complexes. (A) Time course of luciferase degradation in control RL (−HA) and in HA-treated (+HA) RL with and without ATP. Luciferase immunoblots are shown. Amounts of luciferase protein degraded were determined by densitometry. (B) Inhibition of luciferase degradation by hemin and methylated ubiquitin (Met-Ubi) and partial reversal of inhibition by ubiquitin (Ubi) in HA-treated RL. (C) Degradation of luciferase immunoisolated as a chaperone complex from control (−HA) and HA-treated (+HA) RL upon transfer into untreated, degradation-competent RL. Time-dependent degradation is shown in percent of the total luciferase protein in the reaction.