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Southern blot hybridization of duck genomic DNA. Approximately 30 μg of duck genomic DNA was digested overnight with the restriction endonucleases indicated. DNA was resolved on a 1% (wt/vol) agarose gel and transferred to Hybond N membrane (Amersham). The lanes on the right represent 1 and 10 picograms of cloned duck SAA cDNA. The filter was hybridized with a radiolabeled RNA complementary to the 5′ of SAA cDNA (see Materials and Methods).
