Figure 3.

zVAD-fmk blocks DNA fragmentation but not cell death after BAX induction. (A) Jt-Bax-21 cells were incubated with doxycycline (1 μg/ml) for 24 hr in the presence or absence of zVAD-fmk (50 μM). DNA fragmentation (<2N DNA) and cell viability (propidium iodide exclusion) were measured as described (1). (B) Jt-H and Jt-Bax-21 cells were treated with either anti-FAS antibody (100 ng/ml) for 4 hr or doxycycline (1 μg/ml) for 20 hr, respectively, in the presence or absence of zVAD-fmk. The ultrastructure of the cells was analyzed by electron microscopy.