Figure 4.

Reduction of mitochondrial membrane potential (ΔΨm) and production of ROS after BAX induction. (A) Jt-Bax-21 cells were treated with doxycycline for indicated periods of time. Aliquots of 1 × 10⁶ cells were incubated with 50 nM 3,3′-dihexyloxacarbocynine iodide [DiOC₆(3)], 2 μM hydroethidine, or 5 μM DCFH-DA and analyzed by cytofluorometry. The percentages reflect the reduction of ΔΨm [DiOC₆ (3)] (Left), ROS production (hydroethidine converted to ethidum) (Middle), and production of peroxides (DCFH) (Right). Data are representative of three experiments. The open peak in the DCFH panel of 0 hour was a positive control after 10 mM H₂0₂ treatment. (B) Jt-Bax cells were induced with Dox (1 μg/ml) or anti-FAS antibody (100 ng/ml) for 24 hr in the presence or absence of 50 μM zVAD-fmk. The reduction of ΔΨm (Left) and ROS production (Right) were measured as described above.