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Gfi-1 also represses the expression of Bak but does not affect the expression of Bcl-2 in MT-Gfi-1 transgenic thymocytes. Western blots of lysates from thymocytes of MT-Gfi-1 transgenic mice supplied with drinking water without added zinc (−) or containing 25 mM ZnSO4 (+) were probed with antisera to Gfi-1, Bcl-2 (Santa Cruz Biotechnology N-19), or Bak (Santa Cruz Biotechnology G23).
