Figure 3.

Inhibition of both calcium-dependent modes of activation of nuclear transport. Cells were pretreated without (circles) or with (squares) 10 μM thapsigargin as described in Fig. 1 and assayed in the presence of 30 μM calmidazolium and the depleted rabbit reticulocyte lysate. Samples were assayed for the ability to support nuclear transport in the absence (open symbols, broken lines) or presence (closed symbols, solid lines) of 1 mM GTP at various calcium concentrations. The data represent the mean ± SD of two independent experiments.