Table 1.
Inhibition of nuclear transport by calmodulin antagonists
| Antagonist | Relative transport (% of control)
|
|
|---|---|---|
| −GTP | +1 mM GTP | |
| Calmidazolium | −37.6 (10.5) | 118.0 (17.1) |
| Trifluoperazine | 19.2 (16.0) | 130.0 (17.4) |
| Mellitin | −41.3 (5.4) | 68.1 (14.0) |
| W-5 | 75.9 (15.2) | 118.3 (16.8) |
| W-7 | 15.8 (10.6) | 101.8 (34.1) |
| W-12 | 70.4 (21.2) | 117.9 (15.7) |
| W-13 | 9.9 (9.0) | 117.3 (25.3) |
| Calmodulin binding domain | 6.7 (4.3) | ND* |
Antagonists were added to a final concentration of 30 μM to assay incubations containing the depleted rabbit reticulocyte lysate, 79 μM calcium chloride, and either none or 1 mM GTP. Relative nuclear transport was estimated as described in Materials and Methods with the exceptions that a 4°C incubation was used as the negative control, and cytoplasmic fluorescence was subtracted from nuclear fluorescence. Thus, a negative value represents an increased cytoplasmic fluorescence relative to the negative control (4°C incubation). Data represent the mean (SD) for two independent experiments. *ND, no data.