Table 1.
dpm × 10−3 incorporated by
|
||||
---|---|---|---|---|
Brain transglutaminase into
|
Purified
liver transglutaminase into
|
|||
Brain proteins | DRPLA peptide | Brain proteins | DRPLA peptide | |
+ Ca2+ | 1.3 | 22.2 | 11.4 | 548 |
+ EDTA | 0.24 | 6.3 | 0.60 | 5.3 |
Results are expressed as dpm of amine incorporated into 100 μg of protein or peptide. For incorporation by brain transglutaminase, 1.4 mg of brain extract was incubated with or without 100 μg of DRPLA peptide. For incorporation by purified liver transglutaminase, 100 μg of brain extract or of DRPLA peptide were incubated with 11.5 milliunits of the transglutaminase. Reactions were for 2 h at 37°C.