Figure 1.

ICAM-1 promoter activation in 293 cells. (A) Localization of putative binding sites for transcription factors as detected by computer-aided analysis in the 5′-regulatory region of the ICAM-1 gene and in deletion constructs of the ICAM-1 promoter-based luciferase reporter gene constructs. The constructs were named after the number of base pairs relative to the transcription start site. Deletions of interior putative binding sites are indicated by Δ in the construct’s name and within the map. (B) The 293 cells were transiently transfected with the indicated ICAM-1-based luciferase reporter gene constructs and were exposed to 30 J/cm² of UVA radiation (solid bars), 100 J/m² of UVB radiation (gray bars), or sham-irradiation (open bars). ICAM-1 promoter activation was determined as described. Data are given as mean ± SD of relative, specific luciferase activity based on total protein contents and represent four experiments.