Table 2.
Fluorescence-activated cell sorter analysis of ICAM-1 surface expression in cultured human keratinocytes after UVA irradiation
| Additive | ICAM-1 expression, mean fluorescence
intensity
|
||
|---|---|---|---|
| Sham-irradiated | UVA | UVB | |
| None | 6 ± 4 | 120 ± 14 | 140 ± 22 |
| Sodium azide | 20 ± 2 | 10 ± 2 | 140 ± 28 |
| α-Tocopheryl succinate | 15 ± 6 | 10 ± 1 | 130 ± 20 |
| Deuterium oxide | 7 ± 4 | 180 ± 10 | 125 ± 20 |
| Mannitol | 12 ± 6 | 115 ± 20 | 135 ± 8 |
| Catalase | 15 ± 5 | 125 ± 10 | 130 ± 15 |
| Superoxide dismutase | 5 ± 5 | 90 ± 20 | 100 ± 20 |
Keratinocytes were sham-irradiated, UVA-irradiated (30 J/cm2), or UVB-irradiated (100 J/m2) in the presence or absence of sodium azide (50 mM), deuterium oxide, mannitol (0-100 mM, shown for 100 mM), catalase (0-1600 units/ml, shown for 200 units/ml), or superoxide dismutase (0-750 units/ml, shown for 750 units/ml) or preincubated for 24 h with or without α-tocopheryl succinate (25 μM). Cells were harvested 24 h after exposure and were analyzed for ICAM surface expression as described. Data are given as mean ± SD of mean fluorescense intensity of four experiments.