Table 3.

ICAM-1 promoter activation in UVA-irradiated 293 cells

Exposure	Relative, specific luciferase activity per microgram of protein
	pIC277	pIC277ΔNFkB	pIC277ΔAP2
Sham	1.0  ±  0.12	1.0  ±  0.15	1.0  ±  0.18
UVA	3.0  ±  0.59	3.0  ±  0.30	1.1  ±  0.22
+ Sodium azide	1.3  ±  0.19	1.0  ±  0.10	1.3  ±  0.16
+ α-Tocopheryl succinate	1.2  ±  0.24	0.9  ±  0.18	1.1  ±  0.23
+ Deuterium oxide	3.6  ±  0.21	3.8  ±  0.15	1.0  ±  0.30

293 cells were transiently transfected with the indicated ICAM-1-based promoter constructs linked to the luciferase reporter gene, and ICAM-1 promoter activation was determined as described. Cells were either sham-irradiated or UVA-irradiated (30 J/cm²) in the presence or absence of sodium azide (50 mM) or deuterium oxide or after pretreatment with or without α-tocopheryl succinate (25 μM). Data are given as mean ± SD of relative, specific luciferase activity (per microgram of protein) and represent four experiments.