Figure 7.

SirT1 knockdown leads to reduced Thr231-phosphotau. Whole-brain homogenates from 12-month-old 3xTg-AD hemizygous mice (n = 6) and 12-month-old 3xTg-AD mice with 1 copy of SirT1 knocked out (n = 6). A, Western blot analyses show a reduction in SirT1 protein levels and Thr231-phosphotau (AT180) in mice lacking a copy of the SirT1 gene. No changes were seen in total human tau (HT7). B, Quantification of A normalized to actin as a loading control. C, Steady-state levels of acetylated α-tubulin and p25 were not altered in mice lacking a copy of SirT1. D, Quantification of C normalized to actin as a loading control. Error bars indicate SEM, and * indicates significance versus vehicle treatment (p < 0.05).