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. 2009 Jan 12;2008:291808. doi: 10.1155/2008/291808

Figure 4.

Figure 4

Effect of EGCG on the phosphorylation of SAPK/JNK induced by PDGF-BB in MC3T3-E1 cells. The cultured cells were pretreated with the indicated doses of EGCG or vehicle for 60 minutes, and then stimulated by 50 ng/mL PDGF-BB or vehicle for 3 minutes. The extracts of cells were subjected to SDS-PAGE with subsequent Western blotting analysis with antibodies against phospho-specific SAPK/JNK or SAPK/JNK. The histogram shows quantitative representations of the levels of PDGF-BB-induced phosphorylation obtained from laser densitometric analysis of three independent experiments. Each value represents the mean ± SEM of triplicate determinations. Similar results were obtained with two additional and different cell preparations. *P < .05: compared to the value of PDGF-BB alone.