. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Microbes Infect. 2008 Jul 17;10(10-11):1182–1191. doi: 10.1016/j.micinf.2008.06.014

Table 1.

List of primers used in this study

Primers	DNA Sequence (5′ to 3′)^a
Bla F	TCCCCCCGGGCCGCATTAAAGCTTATCGATG^b
Bla R	CGGGATCCCCAATGCTTAATCAGTGAGG^c
igaβ F2	GCTCTAGACCCGAGATACTGACCAAATG^d
igaβ R2	CGGAATTCCAAAGGCATTGAGCTGTAGC^e
igaβ del F	CGGGATCCCCCGGGCTGTTATTCAAGCGAAGATGGGAAGC^f
igaβ del R	CGGGATCCGTACAGCAAAACAATGTGGAAATTGC^g

The underlined sequence represents the restriction enzyme site

Amplifies bla starting 250 bp upstream of bla start codon, XmaI site

Amplifies bla starting at the 3′ end of bla excluding the stop codon, BamHI site

Amplifies iga starting 1095 bp upstream of iga start codon, XbaI site

Amplifies iga starting 94 bp downstream of iga stop codon, EcoRI site

Amplifies sequence starting 149 bp upstream of the iga promoter and continues and continues upstream of iga, BamHI site

Amplifies the β-domain of iga and downstream sequence, BamHI site