Fig. 3.

Minimum Information for Data Analysis in Systems Biology (MIDAS). A: A simplified map of a multi-well experiment in which Akt phosphorylation is to be assayed at 0 and 30 min in extracts from cells treated, or not, with lipo-polysacharide (LPS) and a PI3-kinase inhibitor (PI3Ki). B: MIDAS representation of the experiment. A column header consists of a two-letter code defining the type of column and a short column name. For clarity headers are color-coded to match the corresponding values on the plate map. The leftmost five columns (codes ID: identity, TR: treatment, and DA: data acquisition) are experimental design parameters and would be filled in before bench work begins. The rightmost column holds measured data values (DV) that are appended as data acquisition is performed. See Supplementary Table S2 for a larger example. C: A list of the type codes used for MIDAS columns and a few relevant SBWiki types.