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. Author manuscript; available in PMC: 2009 Jan 12.
Published in final edited form as: Endocrinology. 2006 Nov 16;148(3):1396–1402. doi: 10.1210/en.2006-1109

FIG. 3.

FIG. 3

Effect of VDR level on the hormonal adaptation to variations in dietary calcium intake. Ninety-day-old mice were adapted to diets containing different calcium contents (0.5% Ca, 0.02% Ca, or 2.0% Ca) for a 7-d period before the experiment. Plasma was prepared from blood and analyzed for PTH (A) and 1,25(OH)2 D3 (C) using commercial assays. RNA was isolated from kidneys and analyzed for renal (Kd) CYP27B1 mRNA levels by real time RT-PCR and normalized for the expression of GAPDH (B). Gender was balanced across genotype and diet groups and used as a covariate to correct for previously reported gender differences (35). Values are expressed relative to the WT mice fed the 0.5% Ca diet. Bars represent the mean ± SEM (n = 8-11 for PTH and 1,25(OH)2 D3, n = 6 for CYP27B1). Within a panel, bars with different letter superscripts are significantly different from one another (Fisher's protected LSD, P < 0.05).