Skip to main content
. Author manuscript; available in PMC: 2009 Jan 12.
Published in final edited form as: Epilepsy Res. 2007 Jul;75(2-3):171–179. doi: 10.1016/j.eplepsyres.2007.05.011

Figure 5. Spontaneous recurrent seizures do not cause neuronal cell death.

Figure 5

(A) FDA–PI staining (black bars) and morphological assessment (gray bars) procedures revealed no increase in percent cell death with longer exposure to SREDs. (B) The amount of cell death in SREDs cultures was similar to age-matched control cultures and did not increase with time. Thus there was no increase in neuronal cell death in SREDs phase with time. The cell death in control cultures was around 4–6% and was lower than the control conditions for the pBRS washed cultures due to the lack of washing and handling of the neurons and the direct assessment of the cell death in culture maintained in media without manipulation. (C) Linear regression analysis revealed no significant correlation (r2 = 0.26, p = 0.3) between neuronal cell death and time of exposure to SREDs in cultures.