Fig. 9.

1D gel electrophoresis of O. coriaceus salivary gland homogenates followed by tryptic digestion and HPLC MS/MS of results. Soluble (S) and insoluble (Pellet) salivary gland homogenates of O. coriaceus were dissolved in reducing SDS buffer and applied to a polyacrylamide gel. After electrophoresis, each gel was sliced into 28 (S) or 31 (Pellet) fractions and submitted to tryptic digest and reverse-phase HPLC MS/MS. Numbers on the left indicate molecular weight marker positions in the gel. The bands labeled with Lip X correspond to lipocalins where X is the number of the OC-X protein found in Supplemental Table S2. Similarly, BT is for basic tail proteins, Muc for mucin, Cy for cystatin, DK for double Kunitz, Pl for phospholipase, Mt for metalloprotease, Ctx for cytotoxin-like, 5Nuc for 5′-nucleotidase/apyrase, GR for glycine rich, and U for Unknown protein. Other protein matches can be found in Supplemental Table S2.