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. 2009 Jan 23;5(1):e1000347. doi: 10.1371/journal.pgen.1000347

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Orsi, Tanksley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) TA2080 S/S NIL showing the introgression region from S. pimpinellifolium containing Sw4.1 QTL (shaded). (B) Sw4.1 mapping of F₂-population of 150 individuals (TA209 – L/L NIL×TA2080 – S/S NIL) within a 48 cM region. (C) Sw4.1 mapping within a 11 cM region from 140 individuals from a F₂ heterozygous individual (98T342-95). (D) Sw4.1 mapping within a 2.4 cM region from recombinants selected from 1,000 seeds from F₂ heterozygous individuals. The S1 marker was then used to isolate and sequence the 130 kb BAC clone LE_HBa0077O05. (E) Sw4.1 mapping within the ∼130 kb BAC LE_HBa0077O05 from recombinants selected from 9,000 seeds from F₂ heterozygous individuals. The numbers between markers represent the number of crossover events in each interval.