. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Protein Expr Purif. 2008 Apr 26;60(2):225–231. doi: 10.1016/j.pep.2008.04.006

Table 1.

Representative yield and enzymatic activity of human ALT1 and ALT2 during purification

Fraction	Total protein (mg)		Total activity (U)		Specific activity (U/mg protein)

	hALT1	hALT2	hALT1	hALT2	hALT1	hALT2
Uninfected cell supernatant	0.56	0.56	0.025	0.025	0.04	0.04
ALT lysate	113.8	159	866.6	65.2	7.60	0.41
His-tagged fusion protein	6.12	6.0	329.5	17.6	53.8	3.0
Tag-free recombinant protein	1.38	0.26	83.1	1.1	60.2	4.0