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. 2009 Jan 21;4(1):e4236. doi: 10.1371/journal.pone.0004236

Figure 1. Modulation of Q1/E1 channels by boronates.

Figure 1

(A) Time course of Q1/E1 current measured in ND96 at +40 mV at the end of a 2 s pulse. The current was normalized before compound application. Borax reversibly inhibits while methylboronic acid has little to no effect on channel current. Phenylboronic acid (PBA) initially inhibits current and then slowly potentiates. Benzoic acid reversibly reduces Q1/E1 channel current whereas benzyl alcohol reversibly activates the channel complex. (B) Families of currents recorded before and during treatment with PBA or benzyl alcohol. Currents were elicited by 4 s step test potentials from −80 to +60 mV in 10 mV increments from a holding potential of −80 mV followed by a tail pulse to −30 mV. Dashed line indicates zero current. Scale bars represent 1 µA and 0.5 s. (C) Voltage-activation curves for Q1/E1 calculated from tail current analysis. Solid curves represent Boltzmann fits to the data. Data are presented as the mean±SEM (n = 10).