Table 1. Electrophysiological Properties of KCNQ Channels in the presence of PBA or Benzyl Alcohol.^a .

	Control		Phenylboronic Acid (PBA)					Benzyl Alcohol
	V1/2 (mV)	z	Δ V1/2 (mV)	Δ z	Δgmax	EC50 (mM)	Δ V1/2 (mV)	Δ z
Q1	−31.8±1.6	2.39±0.06	−13.8±1.6*	0.51±0.11	1.5±0.1	0.10±0.02	−4.6±5.5	0.51±0.18
Q1/E1	23.4±1.2	1.70±0.04	−12.6±2.7*	−0.23±0.07	3.0±0.1	1.6±0.5	−4.4±2.4	−0.23±0.07
Q1/E3	26.6±3.0	0.47±0.02	3.3±5.4*	0.33±0.04	3.9±0.2	6.1±5.7	2.7±4.9	0.35±0.06
Q2/Q3	−44.6±1.4	3.08±0.11	−9.8±2.3*	−0.32±0.34	3.0±0.3	3.7±1.4	−5.9±2.9	0.31±0.17
Q4	−15.6±1.1	2.45±0.06	−13.7±1.3*	−0.44±0.11	>10	>10	−0.8±2.9	−0.53±0.08

^aData from individual activation curves obtained from 4–10 oocytes. Activation curves were fit to a Boltzmann function. V_½ is the voltage of half-maximal activation and z is the slope factor. ΔV_1/2 and Δ z are the changes induced by addition of 10 mM compound. Δg_max values were determined during the washout of inhibition, as described in the Materials and Methods. EC₅₀ values were determined during the washout of inhibition for PBA concentrations ranging from 1–10 mM. All values are mean±SEM except for EC₅₀ values, which are reported as the error of the fit to a hyperbola.

^*Indicates significant (Student t-test; p<0.05) when compared to benzyl alcohol.