Figure 3.
Footpad Merkel complexes are not altered in NT4-OE mice. Footpads from wild type (A, C) and NT4-OE (B, D) mice were labeled with anti-cytokeratin 20 to detect Merkel cells (red) and with anti-neurofilament 150 (green) to detect nerve fibers. Merkel cells were organized in clusters subjacent to the epidermis and were innervated in all mice examined. No difference in either the number of Merkel cells or the amount of innervation to Merkel cell complexes was evident. The brightness and contrast was optimized across all images for visualization of both labels. Scale bar in D = 20µm and applies to all.