Figure 5. Release of centromeric heterochromatin from the MAS causes NE and MAS defects.

A. Heh1-GFP is an INM protein that is enriched at the MAS. Micrographs of MKSP47 cells expressing Heh1-GFP and Sad1-DsRed shown with the DIC and merged images. B. A temperature-sensitive mutation in Nuf2 leads to NE and MAS defects. Fluorescence micrographs of cells expressing Heh1-GFP and Sad1-DsRed in the nuf2-1 strain (MKSP109) at 25°C. Sad1-DsRed foci and Heh1-GFP foci are seen in the cytoplasm of nuf2-1 cells (arrows). Quantitation of Heh1 and Sad1 foci in WT versus nuf2-1 cells was determined as in Figure 2. C. The nuf2-1 mutation leads to defects in NE shape. Fluorescence micrographs of strain MKSP109 at 25°C. Quantitation of nuclear shape was carried out as in Figure 2.