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Separation of palmitoylated vs. unpalmitoylated αs by reverse-phase chromatography. (A) Ten picomoles of cytosolic αs-WT, αs-C3S, and membrane-derived αs-WT (purified [3H]palmitoylated αs) were chromatographed on a C4 HPLC reverse-phase column. (B) Radioactivity in column fractions after chromatography (as in A) of purified [3H]palmitoylated αs-WT incubated with (○, 10 pmol of total protein) or without (•, 5 pmol of total protein) palmitoyl esterase.
