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. 2003 Nov 17;1(2):e28. doi: 10.1371/journal.pbio.0000028

Figure 4. A Strain Expressing Pho4 That Cannot Be Phosphorylated on Site 6 Does Not Induce PHO5 in Intermediate-Phosphate Medium.

Figure 4

A strain expressing Pho4WT1234PA6 grown for 2 h in no-, intermediate-, and high-phosphate medium was analyzed by Northern blot analysis (A) and chromatin immunoprecipitation (B). The fold enrichment of PHO5 over ACT1 was 1.71, 2.42, 10.49, 0.99, 1.26, and 2.84 in lanes 1–6, respectively (PHO4WT1234WT6 high, PHO4WT1234WT6 int, PHO4 WT1234WT6 no, wt high, wt int, wt no). The fold enrichment of PHO84 over ACT1 was 24.64, 27.44, 43.74, 1.75, 4.27, and 6.28 in lanes 1–6, respectively.