Figure 10. Slik Acts via Raf to Control Cell Survival and Cell Proliferation.

(A and B) ptc^GAL4 UAS-slik phenotype in different genetic backgrounds.

(A) Quantification of the area between veins 3 and 4 as a function of total wing area. Control: wild-type flies. Other genotypes as indicated.

(B) Detail of the vein 3–4 region in ptc^GAL4 UAS-slik and Raf heterozygous phl^EA75/+; ptc^GAL4 UAS-slik wings. The multiple wing hair phenotype characteristic of Slik overexpression was suppressed when one copy of Raf was removed and the spacing between the veins was reduced.

(C) Survival rates and assessment of the severity of the wing phenotypes in flies of the indicated genotypes.

(D) TUNEL labeling of wing discs to visualize apoptotic cells. Genotypes as indicated. (E–G) slik¹ mutant clones labeled by expression of GFP (green) and by the absence of Slik protein (red). Larval genotypes: UAS-CD8-GFP hsFlp; FRT42 Gal80/FRT42 slik¹; Tub-Gal4/+. (F) Plus UAS-Raf^GOF. (G) Plus UAS-Rl^SEM. Lower panels show optical sections perpendicular to the plane of the epithelium. DAPI (blue) shown alone below.