Figure 3.

Candidate yeast TAFs are associated with TBP. Extracts were prepared from strains carrying epitope-tagged alleles of the indicated proteins and immunoprecipitated with anti-TBP antibodies. The TBP-associated proteins were blotted and probed with the monoclonal antibody recognizing the epitope tag. Lanes: Load, 30 μg of total extract; Im., immunoprecipitate from 300 μg of total extract with anti-TBP; Pre., immunoprecipitate from 300 μg of total extract with preimmune serum. Fus3 serves as a non-TAF negative control.