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Candidate yeast TAFs are associated with TFIID. Extracts were prepared from strains carrying epitope-tagged alleles of the indicated proteins and immunoprecipitated with the 12CA5 monoclonal antibody. To determine whether the candidate yeast TAFs were associated with TFIID, the precipitated proteins were blotted and probed with anti-TAF130. Lanes: L, 15 μg of the indicated extract; IP, immunoprecipitate from 150 μg of extract. Fus3 serves as a non-TAF negative control.
