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Processing of 5C insertion/base substitution mutants in the presence of U75nobp. Lanes 1–6 contain in vitro processing of pre-mRNAs HDEpu→py, wild-type, 5C, 5C+2bp, 5C+3bp, and 5C+5bp, respectively. The same substrates were injected into the following oocytes. Lanes: 7–12, untreated Xenopus oocytes; 13–18, oocytes depleted of Xenopus U7 RNA and rescued with U7wt RNA; 19–24, depleted oocytes rescued with U75nobp RNA; 25–30, depleted oocytes.
