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Substrate specificity of the wt Kit and mutant KDY receptors. Receptors were immunoprecipitated from IC2/Kit and IC2/KDY cells, respectively, with rabbit anti-Kit antiserum and used to phosphorylate the EGFR optimal substrate (EGFS) AEEEEYFELVAKKKK (10 mM), Abl optimal substrate (ABLS) EAIYAAPFAKKKFEAKKK (10 mM), or Src optimal substrate (SRCS) AEEEIYGEFEAKKK (10 mM).
