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. 1996 Dec 10;93(25):14680–14685. doi: 10.1073/pnas.93.25.14680

Figure 4.

Figure 4

PCR amplification of polymorphic Tc1 insertions mapped in high copy number strains. Tc1 flanking fragments were amplified using a primer in Tc1 and a primer in the flanking genomic sequence. In each case, the first lane shows the PCR product using template DNA of the high copy number strain in which the insertion was identified, and the second lane shows the PCR product using Bristol N2 DNA. Markers pkP417 to pkP400 are in RW7000, markers pkP645 and pkP503 are in CB4000, and marker pkP411 is in KR1787. A 1-kb DNA ladder (GIBCO/BRL) was used as a DNA fragment size marker.