Figure 5.

Genetic mapping of dpy-17 and unc-36 using Tc1 STSs. The positions of dpy-17, unc-36, and RW7000 Tc1 STS markers 1–7 on the genetic and physical maps are indicated. The positions of markers 1–7 on the genetic map are extrapolated from the positions of these markers on the physical map. Note that unc-36 has been mapped both to the genetic and physical maps whereas dpy-17 has only been placed on the genetic map. Five pools of 20 Dpy or Unc F₂ animals from crosses to RW7000 were analyzed for crossovers of markers 1–7 by PCR using a primer in Tc1 and unique primers in the flanking genomic sequence of each Tc1 marker. Markers are as follows: 1, pkP417; 2, pkP406; 3, pkP410; 4, pkP402; 5, pkP415; 6, pkP403; and 7, pkP400.