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Vac-Ub-Met-NP-M1 and Vac-Ub-Arg-NP-M1 infected cells produce similar levels of RNA encoding M1 fusion proteins. (a) Uninfected 721.45 cells or (b) 721.45 cells infected with Vac-Ub-Met-NP-M1 (b) and Vac-Ub-Arg-NP-M1 (c and d). Total RNA was extracted from infected cells, separated on a denaturing gel, and transferred to a membrane. The membrane was hybridized with a Ub-Met-NP-M1 DNA probe and autoradiographed. The position of RNA markers in kb is indicated on the right.
