Figure 3.

Inhibition of cell adhesion to vitronectin by anti-αvβ3 mAbs. (A) Adhesion of BW5147 cells to ECM-proteins. BW5147 cells (5 × 10⁴ per well) were incubated in wells previously coated with increasing concentrations of the indicated ECM-proteins. The wells were washed and the remaining cells incubated with MTT. Percent adherence was calculated as described. (B and C) Inhibition of adhesion to vitronectin by anti-αvβ3 mAbs (B) and GRGDSP and GRGESP peptides (C). BW5147 cells were assayed for adhesion to wells coated with vitronectin (1 μg/ml) as described for A, after preincubation with and in the continuous presence of the indicated mAbs or peptides. H-IgM and H-IgG denote irrelevant hamster IgM and IgG mAbs, respectively. One of three independent experiments is shown. Similar results were obtained with wells coated with fibronectin (5 μg/ml) or fibrinogen (10 μg/ml) and when a Vγ1-expressing hybridoma was used instead of the BW5147 cell line.