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Expression of αvβ3 on CD4−CD8− thymocytes. Thymocytes from Rag1-deficient mice were stained with the indicated antibodies as described. The 8-B3, 8-B11, and 1.9 mAbs were used as biotin conjugates and their binding was detected with streptavidin-phycoerythrin. 1.9 is a hamster IgG anti-γδ clonotype mAb used as a negative control.
