Figure 7.

Positions −310 to −57 of the IL-1β promoter are required for LPG’s inhibitory activity. THP-1 cells transfected with plasmid containing positions −3757 to +11 (XT-CAT) or −3757 to −2729 linked to the promoter sequence positions, −131 to +11 (X2-HT-CAT), were treated with LPG (2 μM) (2 h) and challenge with LPS at 2 μg/ml (24 h). Cells were also cotransfected with 2 μg of control plasmid pSV β-galactosidase to normalize for transfection efficiency. After measurement and normalization for β-galactosidase expression, CAT activity were assayed and quantitated (n = 5).