Figure 8.

Time- and dose-dependent effects of LPG on transcription using the IL-1β promoter from positions −310 to +15. (A) Illustrated is the dose-dependent inhibitory activity of LPG on transcription activity (mean of four to eight experiments). (B) Kinetics of LPG treatment on LPS induction of CAT activity (n = 3 to 5 experiments). THP-1 cells transfected with the IL-1 β promoter plasmid were treated either with LPG (0–2 μM) for 2 h or with LPG (2 μM) for indicated time and challenged with LPS (2 μg/ml) for 24 h.