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. Author manuscript; available in PMC: 2009 Jul 25.
Published in final edited form as: J Mol Biol. 2008 May 17;380(5):828–843. doi: 10.1016/j.jmb.2008.05.024

Figure 7.

Figure 7

Endogenous Rac1 activation induced by exogenous expressed wild type Vav1 and Vav1 mutants in HEK293 cells. A) The expression levels of wild type Vav1 and Vav1 mutants in HEK293 cells were detected by Vav1 antibody and oncogenic Vav-1 was detected by HA antibody. B) The expression levels of endogenous Rac1 in HEK 293 cells expressing wild type Vav1 and Vav1 mutants were detected by Rac1 antibody. C) Active Rac1 in HEK293 cells expressing wild type Vav1 and Vav1 mutant was detected by Pull-down assay (described in methods and materials).