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. 1996 Dec 10;93(25):14714–14719. doi: 10.1073/pnas.93.25.14714

Figure 3.

Figure 3

(A) Immunoblot of OCP antigen/β4-integrin (205/210 kDa) using human epidermal, human conjunctival, and tumor cell lines (MDA 435 and UM-SCC-22) and detected by polyclonal antibody to β4-integrin. Lanes: 1, HEL; 2, HCL; 3, MDA 435 cell lysates; 4, UM-SCC-22 cell lysate reacted with polyclonal antibody to β4-integrin; 5–8, same lysates reacted with normal human serum. In all four groups (lanes 1–4), a 205/210-kDa protein band was observed when β4-integrin antibody was used. No similar protein band was detected when normal human serum was used (lanes 5–8). (B) The identical setup of experiment was used except lanes 3 and 4 were UM-SCC-22 and MDA 435, respectively, and monoclonal anti-β4-integrin was used instead of polyclonal.