Figure 1.

Infection of PBLs from LR and HR seronegative individuals with macrophage tropic HIV-1. Heparinized PBLs were collected from HR and LR HIV-negative donors and infected with different cytotropic strains of HIV-1_NL4-3 as described previously (19). (a) Infection of PBLs from two LR (LR-1 and LR-2) and two HR (HR-1 and HR-2) seronegative individuals with HIV_NLAD8 (macrophage tropic, M) at 0.2 moi. Similar result was obtained with the T-cell tropic strain HIV-1_NL4-3 (not shown). (b) Infection of PBLs from LR-1 and HR-1 with HIV_NL4-3 at moi 0.001–0.0001. The moi of 0.005 is equivalent to a TCID₅₀ of 100.