Figure 4.

HIV-1 infection of human lymphocytes in hu-PBL-SCID/bg mice treated or untreated with anti-CD8 antibody. (a and c) FACS analysis of splenocytes from the reconstituted hu-PBL-SCID/bg mouse #11 immunostained with anti-mouse H-2Kd, anti-human CD45, anti-human CD4, or anti-human CD8 as depicted; (b and d) FACS analysis of splenocytes derived from the reconstituted hu-PBL-SCID/bg mouse #14, after anti-CD8 antibody treatment. (e) Infection of SCID/bg mice reconstituted with HR donor 1’s PBLs after CD8 T cell depletion. CD8 T cells were depleted by i.p. injection with 20 μg of purified OKT8 monoclonal antibody five times in total. The reconstituted mice, untreated or anti-CD8 treated as indicated, were infected with HIV-1_NLAD8, and the percentage of infected cells was determined in the spleen and in the peritoneal lavage 2 weeks after virus challenge as described. The mock-infected (mk) mice were not treated with anti-CD8 antibody.