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. 1996 Dec 10;93(25):14736–14741. doi: 10.1073/pnas.93.25.14736

Figure 2.

Figure 2

Expression of activation markers on CD8+ 2C cells is influenced by peptide and costimulation. (a) Expression of CD69 and CD25 on CD8+ 2C cells stimulated with 10 μM peptides presented by transfected Drosophila cells. Purified CD8+ 2C cells were incubated with transfected Drosophila cells plus p2Ca or QL9 peptides (10 μM) in bulk (2 ml) culture for 12 hr and then stained for the markers shown. The data show staining of gated CD8+ cells. (Top) Staining of noncultured 2C cells is shown as a control. (b) Influence of peptide dose on expression of CD25 on CD8+ 2C cells. Purified CD8+ 2C cells were cultured with the indicated concentration of peptides presented by Drosophila cell APC for 12 hr and then stained for CD25 expression on gated CD8+ cells.