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Flagellin-F1-V immunization of BALB/c mice results in a robust antigen-specific IgG response against F1 and V. Groups of seven BALB/c mice were immunized twice i.m. with increasing doses of flagellin-F1-V. Ten days later, the animals were bled and the plasma was evaluated for the levels of anti-F1 and V IgG antibodies by ELISA. (A) Anti-F1 antibody titers; (B) anti-V antibody titers.
