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Evaluation of the sensitivity and specificity of the DENV2 NS1 antigen capture ELISA for the detection of NS1 in cell cultures. Serial dilutions of supernatants obtained from virus cultures infected with the four DENV serotypes were subjected to the DENV2 NS1 antigen capture ELISA. Positive OD450 values were only observed for the DENV2 serotype (the data points for the other serotypes overlap those of the control).
