Comparison of inactivation of Clostridium botulinum FRRB 2802 in MPA3679 agar by heat-only and HPT processing. ▪, heat-only inactivation at 105°C; □, HPT inactivation at 600 MPa and 110°C for 180 s. F105°C values for HPT data were based on a z value of 10.5°C (r2 = 0.98), derived from heat-only inactivation of C. botulinum FRRB 2802 in MPA3679 agar at 105°C (D105°C = 12.7 min; r2 = 0.94), 110°C (D110°C = 3.0 min; r2 = 0.95), and 118°C (D118°C = 0.7 min; r2 = 0.93). Heat-only inactivation data are means of two replicates, and error bars are standard deviations. HPT inactivation data are for independent samples.