FIG. 1.
Reproducible quantitative DNA recovery of oomycete and algal DNA from infected samples. (A) The reproducibility of DNA recovery was tested with Laminaria digitata gametophytes infected with Eurychasma strain CCAP 4018/1 or CCAP 4018/2. The DNA of two to four independent biological replicates was extracted either with a commercial kit (Qiagen) or by a CTAB-based protocol (CTAB) and used for qPCR with the primers CG60/CG61 (Eurychasma oligonucleotides) and CG64/CG65 (Laminaria oligonucleotides). (B) ΔCT values [calculated as (CT)Eurychasma − (CT)Laminaria] deduced from panel A.