FIG. 2.
qPCR determination of differential susceptibility of Ectocarpus strains to Eurychasma. Coincubation of Ectocarpus strains with Eurychasma strain CCAP 4018/2. CCAP 1310/4 and CCAP 1310/13 were used as susceptible and resistant controls, respectively. All other algal strains surveyed in this experiment exhibited disease symptoms. ΔCT [calculated as (CT)Eurychasma − (CT)Ectocarpus] was negatively correlated with the severity of symptoms observed microscopically, closely reflecting the variation in symptom intensity between strains. Values represent means ± standard deviations of two replicates. The small amounts of Eurychasma DNA detected in the resistant control are attributed to the inoculum.